Under the umbrella of Pathology, the lab section that I supervise has two main functions: necropsy and histopathology.
Histopathology refers to both the study of disease in tissues and the process of preparing tissue samples so they can be examined under a microscope. Tissue samples are systematically collected during necropsies. Histopathology is one of the many tools we use to help determine the cause of death of an animal. Veterinarians (and doctors) also remove tissues during surgical biopsies and submit those for histopathology. In those cases, we are not necessarily looking for the cause of death but examining the tissues to determine if cancer is present, or to identify the cause of a persistent lesion or disease state in the animal.
Tissues slated for histopathological analysis must first be fixed. The most common fixative is formalin, an aqueous solution of formaldehyde. Formalin causes cross-links to form between proteins and makes tissues stiff. Formalin-fixed tissues don’t decay (they can degrade over time, but we will let this oversimplification go). Once the tissues are stiff, they can be cut into very thin slices and placed on microscope slides. The rule of thumb for proper fixation of a tissue sample is a 10:1 ratio of formalin to tissue.
But this post isn’t about what goes on in a histopathology lab. It’s about the containers that veterinarians choose to hold tissue samples and formalin that they then ship to us. I keep pictures of the more creative choices in a folder that I labeled “Sample Wall of Shame.” Here are some of the best.
This container is for food, human food.
So is this one. It was sealed with packing tape and formalin leaked out during transport.
This vet submitted the uterus of a dog in a Gold Peak tea bottle. When collected at the time of surgery, the tissue undoubtedly went into the bottle easily. We had to cut the bottle apart to get the fixed tissue out. And yes, that is a zip tie that was used to close off part of the uterus. We still don’t know why.
This vet chose a pill bottle which does not have a liquid-proof seal. They thoughtfully folded the submission form into the same baggie as the pill bottle. Formalin leaked onto the submission form.
This vet chose a glass mason jar and opted to seal it with packing tape instead of wax film. Glass is an insanely terrible choice. And the submitter still didn’t have enough formalin for the size of the sample.
This vet used an appropriately sized, commercially prepared jar with sufficient formalin to fix the submitted tissue samples. They had marked one of the samples with a suture, a common way to differentiate multiple samples placed into one jar. Unfortunately, the suture caught in the threads when the jar was closed and most of the formalin leaked out during transport, contaminating other samples in the same shipping box.
There was not enough formalin in this commercially prepared jar for this large tissue sample.
Why does any of this matter? Formalin can leak out of poorly sealed containers and contaminate submission forms, packing materials, and other samples in the same shipping box. Leaks expose shipping, receiving, and histopathology personnel to formalin, a carcinogen. Tissues that don't get fixed completely can rot, obscuring diagnostic details. Glass containers can break, creating sharps hazards.
Sure, the commercially prepared jars that I showed in the last two examples cost money and some vets may want to cut costs where they can. But selecting any old container that comes to hand creates unnecessary risks, potentially ruins samples, and shows poor attention to detail and poor understanding of the requested diagnostic test.
To be sure, this is not a problem unique to our lab. All diagnostic labs have to deal with improperly packaged samples. We wear PPE and use biological safety cabinets and fume hoods to handle samples to mitigate risks on our end. And of course we educate clients who submit samples like this as often as we can.
Even so, I regularly add photos to my Sample Wall of Shame folder.